Antigen

Antigen Class

TFs and others

Antigen

Elk1

Cell type

Cell type Class

Neural

Cell type

Corpus Striatum

MeSH Description

Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.

Sample

source_name

striatum

model

R6/1

age

8 weeks

tissue

Striatum

chip antibody

Elk-1 (Santa Cruz, sc-355X)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked tissues were fragmented to the size range of 100–500 bp using a Bioruptor (Bioruptor Next Gen; Diagenode). Antibodies that specifically recognizes H3K27 acetylation (Abcam, ab4729) and Elk-1 protein (Santa Cruz, sc-355X) along with nonspecific rabbit IgG (Millipore, 17-614) were incubated with beads for 6 h before incubating with sonicated chromatin overnight. Immunoprecipitated DNA and nonspecific IgG-bound DNA were prepared for high-throughput sequencing according to Illumina's instructions and sequenced on an Illumina platform following the manufacturer's standard protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

96974821

Reads aligned (%)

99.6

Duplicates removed (%)

51.9

Number of peaks

4446 (qval < 1E-05)

mm9

Number of total reads

96974821

Reads aligned (%)

99.5

Duplicates removed (%)

51.9

Number of peaks

4341 (qval < 1E-05)